Before doing ANYTHING, please contact Dr. Kelly Higgins by phone at 505-272-4663 or by email at KJHiggins@salud.unm.edu for ADVICE on the PROCEDURES and a FLOW CHART telling you the STEPS REQUIRED in obtaining tissue.
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This comprises cutting sections of frozen or paraffin-embedded formalin fixed tissues.
The tissue can be stained with an H&E stain or remain unstained.
The laboratory can stain tissue sections with standard antibodies using protocols already in place. Alternatively, the Laboratory can work up antibodies using the VENTANA DISCOVERY, which is a new platform allowing multiple staining combinations to optimize staining results.
Fluorescence-tagged antibodies can also be used to detect your protein of interest.
Tissue microarray design and construction: This entails collecting donor paraffin blocks, selecting the areas of interest to core, and placing the cores in a blank recipient paraffin block. The advantages are in speed and cost, as multiple samples may be assessed on one slide. The typical TMA contains approximately 30-50 cores of 1 -2.0 mm diameter. Approximately 50 to 200 sections may be obtained from one TMA block.
Archival Tissue Retrieval:
The HTR-TASR personnel can retrieve archival tissue blocks from UNM and Presbyterian Hospitals.
Prospective Specimen Collection:
This usually comprises collection of excess or remnant tissues left over at the time of surgical resection after diagnostic samples have been taken for surgical pathologist microscopic review and enough specimen is retained in paraffin for potential future testing for patient care.
HTR-TASR personnel will collect the tissues in the Gross Dissection Room of UNM HSC Surgical Pathology. Currently, the HTR does not perform venipunctures but will bank such specimens as requested by researchers.
The HTR-TASR technical team collects specific areas on a tissue section. This can be done manually by a pathologist. The method of collection destroys cell morphology and is thus suitable for morphology independent projects only.
Clinical and Pathology De-identified Data Retrieval:
HTR-TASR project consultants can collect pathology and clinical data that are stripped of HIPAA identifiers while coded to matching samples.